YIp357R


Vector IG Sequence Link :
General : plasmid ds-DNA 7102 BP
Functions : (cloning)
Selection : ()
Copy Number :
Hosts : (E.coli HB101)(Saccharomyces cerevisiae)(E.coli)(E.coli MC1061)
Suppliers : (ATCC)
Misc.Comments : Created by Moore, July 1995, under contract with NCBI. The sequence and reading frame of the multiple cloning sequence is: 5' A AGC TTG CAT GCC TGC AGG TCG ACT CTA GAG GAT CCC CGG GTA CCG AGC TCG AAT TCC CAG CTT GCT CCC3', from nucleotide 1 of MCS through CCC for amino acid 8 of beta-galactosidase. One of 3 promoter-cloning, YI type shuttle vectors (ATCC 37755 to ATCC 37757) with URA3 selection in Saccharomyces cerevisiae, a beta-galactosidase reporter gene and multiple cloning sites differing in reading frame. The cleavage position in the reading frame for cloning sites is (where 3 = between triplets): HindIII-3; SphI-1; PstI-1; SalI-3; XbaI-3; BamHI-3; SmaI-1; KpnI-1; SacI-1; EcoRI-3. The SacI site is not unique. Restriction digests of the clone give the following sizes (kb): EcoRI--7.1; SalI--7.1; PstI--7.1; HindIII--7.1. (ATCC staff) Medium is 1227 LB plus ampicillin.
Parents : (YEp353)(YIp352)
Siblings : ()
Descendents : ()


Return to Vector Homepage