YIp366


Vector IG Sequence Link :
General : plasmid ds-DNA 7000 BP
Functions : (cloning)
Selection : ()
Copy Number :
Hosts : (E.coli HB101)(Saccharomyces cerevisiae)(E.coli)(E.coli MC1061)
Suppliers : (ATCC)
Misc.Comments : Restriction digests of the clone give the following sizes (kb): EcoRI--4.1, 2.9; SalI--7.0; PstI--7.0; HindIII--7.0. (ATCC staff) The sequence and reading frame of the multiple cloning sequence is: 5' GA ATT CGA GCT CGG TAC CCG GGG ATC CTC TAG AGT CGA CCT GCA GGC ATG CAA GCT TGC GAT CCC 3', from nucleotide 1 of MCS through CCC for amino acid 8 of beta-galactosidase. One of 3 promoter-cloning, YI type shuttle vectors (ATCC 37752 to ATCC 37754) with LEU2 selection in Saccharomyces cerevisiae, a beta-galactosidase reporter gene and multiple cloning sites differing in reading frame. The cleavage position in the reading frame for cloning sites is (where 3 = between triplets): EcoRI-2; SacI-3; KpnI-3; SmaI-2; BamHI-2; XbaI-2; SalI-2; PstI-3; SphI-3; HindIII-2. The EcoRI, SacI, and KpnI sites are not unique. Cloning into the EcoRI, SacI, KpnI SmaI, BamHI, or XbaI sites leads to a TAG stop codon within the downstream XbaI site of the multiple cloning region. Medium is 1227 LB plus ampicillin.
Parents : ()
Siblings : ()
Descendents : ()


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