Vector IG Sequence Link :
General : plasmid ds-DNA 5541 BP
Functions : (cloning)
Selection : ()
Copy Number :
Hosts : (E.coli K-12 r-m+)(E.coli C600)(E.coli MM294)(E.coli)(S.cerevisiae)
Suppliers : (NEB)(ATCC)
Misc.Comments : These data and their annotation were supplied to GenBank by Will Gilbert under the auspices of the GenBank Currator Program. The length of G- or C-tails used in the cloning procedure is not exactly known. Therefore the sequencea 1428-1437, C(10) and 2601-2610 G(10) are tentative. Size could be 5541 bp. ATCC size is 5500 bp. This was constructed by cloning the 1.1 kb HindIII fragment containing URA3 from lambda590-Sc2904 into the AvaI site of pBR322. The 5' - 3' orientation of URA3 is clockwise; SmaI & AvaI sites in the 3' of URA3 are close to the PvuII site of pBR322. [7] A general purpose integrating Saccharomyces cerevisiae/Escherichia coli shuttle vector. [1] Medium is 1227 LB plus ampicillin.
Parents : (pBR322)(2micron)
Siblings : ()
Descendents : ()


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