YIp5-32cat


Vector IG Sequence Link :
General : plasmid ds-DNA 6610 BP
Functions : (cloning)
Selection : ()
Copy Number :
Hosts : (E.coli RR1)(Saccharomyces cerevisiae)(E.coli)
Suppliers : (ATCC)
Misc.Comments : The depositor recommends using YRp7-17camp (ATCC 37630) as the reference for CAT expression and using the CAT assay methodology of Neumann, et al. One of a series (ATCC 37624 through 37634) of Escherichia coli/Saccharomyces cerevisiae shuttle vectors for investigating control of expression in yeast. A 5195 bp BamHI/HindIII fragment of YIp5 was ligated to a 1415 bp HindIII/BamHI fragment of YRp7-32cat (ATCC 37624). Restriction digests of the clone give the following sizes (kb): EcoRI--5.8, 0.64, 0.58; PstI--3.85, 3.20; PvuII--3.7, 3.25. (ATCC staff) Medium is 1227 LB plus ampicillin.
Parents : ()
Siblings : ()
Descendents : ()


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