YpGX265GAL4


Vector IG Sequence Link :
General : plasmid ds-DNA 12300 BP
Functions : (cloning)
Selection : ()
Copy Number :
Hosts : (E.coli)(Saccharomyces cerevisiae)(S.cerevisiae 442)(S.cerevisiae SHY1)
Suppliers : (ATCC)
Misc.Comments : High copy number YE-type shuttle expression vector. [1] The promoter consists of nt -610 to -157 of the GAL 1-10 upstream activation site,fused to the transcription initiation site (nt -158 to -1) of the alpha mating factor gene MFalpha1. [1] The 3.65 kb HindIII fragment encodes the positive regulator protein GAL4, necessary for transcription from the GAL1-10 promoter. [1] The PHO5 signal sequence (51 nt) was modified to use preferred Saccharomyces codons. [1] The plasmid is maintained at approximately 200 copies per cell in haploid S. cerevisiae cells. [1] The order of the major features in the plasmid is: HindIII - GAL4 - HindIII - Gal1-10 UAS - MFalpha1 transcription initiation sequence - PHO5 signal sequence - EcoRV - BamHI - GAPDH terminator - ampR - LEU2-d - 2 micron ori. [1] Restriction digests of the clone give the following sizes (kb): EcoRV--5.1, 4.2, 3.0; BamHI--8.1, 4.2; HindIII--8.5, 3.8. (ATCC staff) Medium is 1227 LB plus ampicillin.
Parents : ()
Siblings : ()
Descendents : ()


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