YRp14-his3delta200


Vector IG Sequence Link :
General : plasmid ds-DNA 11200 BP
Functions : (cloning)
Selection : ()
Copy Number :
Hosts : (E.coli HB101)(Saccharomyces cerevisiae)(E.coli)
Suppliers : (ATCC)
Misc.Comments : A 1.0 kb region that encompasses the entire coding region of HIS3 was deleted from a genomic EcoRI/SalI fragment (final size=5.1 kb) cloned into the EcoRI/SalI sites of YRp14. [1] A marker addition/transplacement vector. [1] Restriction digests of the clone give the following sizes (kb): BamHI--11.0,0.5; PstI--6.1, 4.0, 1.2; BglII--10.0, 1.15; KpnI--11.5. (ATCC staff) Non-integrants grow poorly selecting for Ura3+ (pinpoints at 5 days) while integrants grow well because the SUP11 copy number is maintained at one. If there are no integrants on a transformation plate, streak onto -URA plates and pick papillae. (personal communication) Medium is 1227 LB plus ampicillin.
Parents : ()
Siblings : ()
Descendents : ()


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