YRp14-trp1delta63


Vector IG Sequence Link :
General : plasmid ds-DNA 8400 BP
Functions : (cloning)
Selection : ()
Copy Number :
Hosts : (E.coli HB101)(Saccharomyces cerevisiae)(E.coli)
Suppliers : (ATCC)
Misc.Comments : A marker addition/transplacement vector. Constructed by inserting the equivalent of 2 genomic EcoRI/HindIII fragments (1.5 kb, centromere proximal and 0.8 kb, 3' end) from the TRP1 region into YRp14. Represents the deletion of a 0.6 kb EcoRI/HindIII fragment containing the promoter and the 5' portion of the gene. Non-integrants grow poorly selecting for Ura3+ (pinpoints at 5 days) while integrants grow well because the SUP11 copy number is maintained at one. If there are no integrants on a transformation plate, streak onto -URA plates and pick papillae. (personal communication) Restriction digests of the clone give the following sizes (kb): EcoRI/HindIII--6.0, 1.6, 0.6; HindIII--8.4; BamHI--8.4; PstI--3.7, 3.4, 1.3; BglII--7.4, 1.1. (ATCC staff) Medium is 1227 LB plus ampicillin.
Parents : ()
Siblings : ()
Descendents : ()


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