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Download data:

Data for the figures:
Data for the clusters presented within the paper are available here as text tab-delimited cluster files. These downloadable data files can be viewed on IBM PC-compatible computers using Treeview, a publicly available software package written by Michael Eisen.

The data contained in all files represents the normalized, background-corrected log2 values of the Red/Green ratios measured on the DNA microarrays.

Data from the figures have been mathematically transformed as described in Materials and Methods, such that each sample described is compared to the unstressed cells. The untransformed data can be accessed in the Complete Dataset file, with descriptions of each microarray reference in the online version of Materials and Methods.


** Misalignment of the MSN2/4 overexpression data in the Complete Dataset has been corrected.


Raw data:
Raw data for all yeast genome microarrays used in this study are available here. For each microarray, two scan files (TIF files) were generated, one for each fluorescence emission wavelength corresponding to the fluorophor used in the reverse transcription labeling reaction (i.e. Cy3-dUTP = 532 nm, Cy5-dUTP = 632 nm). Image analysis for each array was performed on IBM PC-compatible computers using ScanAlyze, and included a semi-manual gridding process whereby the spots were identified in a grid file. The extracted data files are available as compressed text tab-delimited ScanAlyze output files. These files can be viewed directly on the Stanford Microarray Database

The data for each spot on the array are displayed in each row of the Data file. Column headers indicate the green (CH1) and red (CH2) signal intensity (I), background (B), and background corrected intensity (D) for each spot, including the normalized values for CH2 signals (N). The normalized, relative transcript levels (R/G ratios) are represented in log2 space (RAT2N).



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