Data for the figures:
Data for the clusters presented within the
paper are available here as
text tab-delimited cluster files. These downloadable data files can be
viewed on IBM PC-compatible computers using Treeview, a publicly
software package written by Michael Eisen.
The data contained in all files represents the normalized, background-corrected log2 values of the
ratios measured on the DNA microarrays.
Data from the figures have been mathematically transformed as described in Materials and
that each sample described is compared to the unstressed cells. The
untransformed data can be accessed in the Complete Dataset file, with
descriptions of each microarray reference in the online version of Materials and Methods.
** Misalignment of the MSN2/4 overexpression data in the Complete Dataset
has been corrected.
Raw data for all yeast genome microarrays used in this study are available here. For each microarray, two scan
files (TIF files) were generated, one for each fluorescence emission
corresponding to the fluorophor used in the reverse transcription labeling
reaction (i.e. Cy3-dUTP = 532 nm, Cy5-dUTP = 632 nm). Image analysis for
each array was performed on IBM PC-compatible computers using ScanAlyze,
and included a
semi-manual gridding process whereby the spots were identified
in a grid file. The extracted data files are
available as compressed text tab-delimited ScanAlyze output
files. These files can be viewed directly on the Stanford
The data for each spot on the array are displayed in each row of the Data
file. Column headers indicate the green (CH1) and red (CH2) signal intensity (I),
background (B), and background corrected intensity (D) for each spot, including the
normalized values for CH2 signals (N). The normalized, relative transcript levels (R/G ratios)
represented in log2 space (RAT2N).