|
Home
|
|
Yeast mRNA translation homepage
|
|
Figures
|
|
Figures in the paper
|
|
Data
|
|
Interactively explore and download the data
|
|
Supplement
|
|
Supplemental information
|
|
Protocols
|
|
Supplemental protocols
|
|
Authors
|
|
People involved in the project
|
|
|
|
Data - Search
|
Global
yeast ribosomal association profiles
- Search
individual yeast ribosome loading profiles by
either Gene ID or Name.
- Browse
the global yeast ribosome loading
profiles
-
Data in a tabulated format, as well as an off-line searchable browser, are
available in the downloads section below.
Each mRNA has displayed a gif image of its ribosome loading
profile. Each profile contains data from three independent experiments
(indicated by small symbols) and the average values for each fraction
indicated by blue diamonds connected with a line. Black numbers on the
x-axis are the fraction numbers and the red numbers indicate the
number of bound ribosomes. Ribosome occupancy, mRNA transcript length,
number of ribosomes, ribosome density values, and peak fractions are
listed at the top of each gif image.
|
|
|
Data - Download
|
|
The following files are available as
Excel spreadsheets. If you click the link, the file will
open in your web browser. To save the file to your computer,
please use the "Save link as..." or "Save target as..."
feature of your browser. Windows users right-click the link,
and Mac users control-click or click-and-hold the
link.
| File |
Size |
Description |
All primary data
ZIP |
8.9 MB
(Excel)
3.2 MB
(ZIP) |
Table in Excel format containing the primary data for all genes from all
experiments (total of 42 arrays), expressed either as
log2 ratios or as ratios. Excluded spots are
those with abnormal morphology, pixels regression
correlation <0.6 or intensity too close to the
background (i.e. spot intensity < 2.5 times
background intensity). |
Polysome Profile Browser
Excel
ZIP |
7.2 MB
(Excel)
3.5 MB
(ZIP)
|
Image Display (version 0.92y), an image polysome profile browser containing
the polysome profiles and translation features for 5,701 yeast ORFs. This program is based on Microsoft Excel Visual Basic macros; enable macros upon opening the program. This program is compatible only with the Windows 9x/NT/2K operating systems.
|
| Data Summary |
2.4 MB (Excel) |
Excel file
containing a summary of all the data for the 739, 2128, and
5701 subsets of genes including: Name, Description,
length, occupancy, density and abundance. The assignments
of peak fraction from bootstrap analysis are also
included in this file.
|
| EDTA Treatment |
1.5 MB (Excel) |
Results of microarrays
analysis of EDTA treated polysomes. Polysomes collected
and separated on sucrose gradients in the presence of 10
mM EDTA. The gradient was fractionated to 7 fractions and
fractions 1, 3, 5, and 7 were hybridized to 4
microarrays. For 5273 genes, the normalized ratios in
each of the microarrays are presented. The fraction in
the polysomes for each mRNA was calculated by summing the
ratios in the polysomal fractions (fractions 4 to 7) and
dividing by the sum of all fractions. Since fractions 2,
4, and 6 were not analyzed by microarray, their values
were estimated by averaging their nearest
neighbors.
|
|
|
Data for individual microarrays are publicly available for download through the Stanford Microarray Database.
You can access the raw microarray data by clicking
here.
|
|
