A) Polysomal profiles from DNA microarray analysis and Northern blots. The upper panel for each gene is the profile obtained from the microarray analysis and the lower panels are profiles from Northern blots. For each gene, the sample intensity in each fraction has been color coded and aligned horizontally (color scale shown below). Gray squares represent values that did not pass the quality selection criteria; to allow normalization these fractions were assigned to have the averaged intensity of their neighbors. For PMA1, fractions 1-5 were not analyzed by Northern blot. ORF length is indicated below each gene. B) Sample polysomal profiles from triplicate DNA microarray analyses. The smaller symbols represent data from individual experiments, and the blue diamonds connected by a line are the average values for each fraction. The percent of total mRNA for the given gene in a given fraction is plotted. Black numbers on the x-axis are the fraction numbers and the red numbers indicate the number of bound ribosomes. Ribosome occupancy and ribosome density values (see text) are indicated for each gene. Analogous plots for all genes are available on the Web supplement.