Supplemental Table 4: Average number of ribosomes in each
fraction
|
| Fraction
|
Exp. 1
|
Exp. 2
|
Exp. 3
|
| 6
|
1
|
1
|
1
|
| 7
|
1
|
1
|
1
|
| 8
|
2
|
2
|
2
|
| 9
|
3
|
3
|
3
|
| 10
|
4.5
|
4.5
|
5
|
| 11
|
7 (5.7-8.8)
|
7 (6-8)
|
7 (5.8-8.5)
|
| 12
|
11.5 (8.8-13.6)
|
10 (8.1-12.7)
|
10.5 (8.5-12.9)
|
| 13
|
16 (13.6-17.6)
|
15.5 (12.7-18.7)
|
16.5 (12.9-19.9)
|
| 14
|
24 (17.6-30.4)
|
23.5 (18.7-28.4)
|
24 (19.9-28.7)
|
|
|
The number of ribosomes in fractions 6 to 11 was assigned according to the OD 254 peaks
in each fraction. Numbers in parentheses indicate the range of ribosomes in this fraction. For fractions
12 to 14, the numbers were estimated by plotting the distance from the center of centrifugation of the
distinct peaks (up to 8 ribosomes) and extrapolating from the derived logarithmic curve fit. The plots
for the three experiments are presented below. Symbols represent the measured distances and the
black line indicates the logarithmic curve fit. Notes that in all
experiments R2 ³ 0.985.
|
 |